General Cell Based Assays
Eurofins Discoverx cAMP Hunter eXpress ADCYAP1R1 (PAC1) CHO-K1 GPCR Assay, 2pl Kit. (95-0064E2CP2M)
The cAMP Hunter ADCYAP1R1 (PAC1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. PACAP) based activation of ADCYAP1R1 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type ADCYAP1R1, a naturally Gs coupled GPCR. Upon activation of ADCYAP1R1, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical PhagocytosIs asy KItIgG P 1ea
Cayman’s Phagocytosis Assay Kit (IgG PE) employs latex beads coated with fluorescently-labeled rabbit IgG as a probe for the measurement of the phagocytic process in vitro. The engulfed fluorescent beads can be detected using a fluorescence microscope, allowing kinetic studies of phagocytosis at the single-cell level. In addition, the flow cytometric readout provides the advantage of visualizing perturbations in phagocytosis on the population level and, when combined with antibody staining, of specific cell types within complex populations. This kit provides enough Latex Beads-Rabbit IgG-PE Complex for up to 750 samples.
Revvity Health Sciences Inc HTRF HIV p24 Detection Kit, 500 Assay Points
HTRF HIV p24 Detection Kit, 500 Assay Points
Eurofins Discoverx KILR Detection Kit, 1,000 dp (96-well). (97-0001M)
The KILR Detection Kit is a detection kit intended for use with KILR cell lines or pools when running ADCC or other lysis-based assays. This kit contains exogenous EA as well as EFC substrate to allow for detection of lysis-based release of ProLabel-tagged KILR reporter protein. This kit contains all components required to assay 10 plates (96-well). For detailed product information, User manual and COA, visit www.discoverx.com/products/reagents/detection-kit-97-0001m.
Eurofins Discoverx PathHunter eXpress rVIPR1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0913E2RCP2L)
The PathHunter rVIPR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. VIP) based activation of rVIPR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged rVIPR1 and EA-tagged β-Arrestin-2. Activation of rVIPR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress cyno CXCR1 HEK 293 β-Arrestin GPCR Assay, 10pl Kit. (93-0980E1CCP0L)
The PathHunter cyno CXCR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL8/IL8) based activation of cyno CXCR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged cyno CXCR1 and EA-tagged β-Arrestin-2. Activation of cyno CXCR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Cynomolgus monkey version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress OSMRb/IL31RA Dimerization Assay, 2pl Kit. (93-1002E3CP5M)
The PathHunter OSMRb/IL31RA Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-31) induced dimerization of OSMRb/IL31RA receptors. This product uses EFC technology. The cell line included overexpresses OSMRb target protein fused to PK alongside IL31RA target protein fused to EA. Upon ligand-induced dimerization of OSMRb/IL31RA, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress UTR2 Total GPCR Internalization Assay, 2-Plate Kit. (93-0746E3CP5M)
The PathHunter UTR2 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of UTR2 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged UTR2 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the UTR2 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CHRM1 U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0859E3CP0M)
The PathHunter CHRM1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. acetylcholine) based activation of CHRM1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CHRM1 and EA-tagged β-Arrestin-2. Activation of CHRM1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Selleck Chemical LLC Smoothened Agonist (SAG) HCl 2mg 2095432-58-7
Smoothened Agonist (SAG) HCl is a cell-permeable Smoothened (Smo) agonist with EC50 of 3 nM in Shh-LIGHT2 cells. *For Research & Development use only. Product is not intended for drug, household, or human consumption.
Selleck Chemical LLC KC7F2 20mg 927822-86-4
KC7F2 is a selective HIF-1? translation inhibitor with IC50 of 20 ?M in a cell-based assay. *For Research & Development use only. Product is not intended for drug, household, or human consumption.
Eurofins Discoverx PathHunter eXpress HCRTR1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0258E2CP0L)
The PathHunter HCRTR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. orexin A) based activation of HCRTR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HCRTR1 and EA-tagged β-Arrestin-2. Activation of HCRTR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress EDNRA CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0257E2CP0L)
The PathHunter EDNRA eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. endothelin-1) based activation of EDNRA GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged EDNRA and EA-tagged β-Arrestin-2. Activation of EDNRA stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.