General Cell Based Assays
Revvity Health Sciences Inc HTRF Human & Mouse PARP cleaved-Asp214 Detection Kit, Control Lysate
HTRF Human & Mouse PARP cleaved-Asp214 Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-VASP (Ser157) Detection Kit, Control Lysate
HTRF Human & Mouse Phospho-VASP (Ser157) Detection Kit, Control Lysate
Abcam Mevastatin, HMG-CoA reductase inhibitor, 50MG
MW 390.5 Da, Purity >98%. Potent HMG-CoA reductase inhibitor. Proapoptotic, able to arrest cells in G₁ by down-regulation of CDK4, CDK6 and cyclin D1 and up-regulation of p21 and p27.
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress CCR4 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0193E2CP0M)
The PathHunter CCR4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL22/MDC) based activation of CCR4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCR4 and EA-tagged β-Arrestin-2. Activation of CCR4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical PAF ACtylhydrolase asy KIt 96
PAF-AH catalyzes the hydrolysis of the potent biologically-active phospholipid PAF, generating inactive lyso-PAF. Cayman’s PAF-AH assay kit provides an accurate and convenient method for measurement of PAF-AH activity. The assay uses 2-thio PAF which serves as a substrate for PAF-AH. Upon hydrolysis of the acetyl thioester bond at the sn-2 position by PAF-AH, free thiols are detected using Ellman’s reagent.
Eurofins Discoverx PathHunter eXpress cyno CXCR1 HEK 293 β-Arrestin GPCR Assay, 2pl Kit. (93-0980E1CCP0M)
The PathHunter cyno CXCR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL8/IL8) based activation of cyno CXCR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged cyno CXCR1 and EA-tagged β-Arrestin-2. Activation of cyno CXCR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Cynomolgus monkey version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical Cysteinyl Leukotriene Express
Cayman’s CysLT Express ELISA Kit is a competitive assay that can be used for quantification of CysLTs in urine, plasma, serum, lavage fluids and aspirates, culture media, and other sample matrices. The assay has a range of 7.8-1,000 pg/ml, an average sensitivity (80% B/B0) of 24 pg/ml, and a lower limit of detection (LLOD) of 6.4 pg/ml. NOTE: This kit measures all CysLTs. For measurement of specific CysLTs, please see Cayman Chemical’s Leukotriene E4 ELISA Kit (Item No. 501060) and Leukotriene C4 ELISA Kit (Item No. 501070).
Eurofins Discoverx AssayComplete Cell Culture Kit-110, (92-3110G)
AssayComplete Cell Culture Kit-110 is an optimized media kit intended for use in culturing and propagating Eurofins DiscoverX cell lines. This kit contains the base medium, relevant serum, and pen/strep required to produce a full cell culture medium for optimal cell culture results. This product is provided as a 3-component kit which will produce approximately 550 mL of finished media. Please refer to cell line specific datasheet to determine the recommended cell culture kit. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress GPR97 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0410E2ACP1L)
The PathHunter GPR97 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR97 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR97 and EA-tagged β-Arrestin-2. Activation of GPR97 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx Calcium No WashPLUS, 10 plates (384-well). (90-0091)
The Calcium No WashPLUS detection kit is intended for use with cells expressing receptors that signal through calcium. The kit contains a calcium-sensitive dye which assays intracellular calcium levels upon activation of a Gαq-coupled GPCR. Activation of the GPCR leads to release of intracellular calcium, and a detectable increase in fluorescent signal. This detection kit contains reagents for assaying 10 plates. For detailed product information, User Manual and datasheet, visit www.discoverx.com/products/cell-line/calcium-no-washplus-90-0091.
Abcam HDAC substrate, colorimetric (Boc-Lys(Ac)-pNA), 25MG
MW 408.45 g/mol, Purity >98%. Colorimetric substrate for histone deacetylases (HDAC). For use in HDAC activity assays.
The product is subject to the following: Abcam Restricted Use Statement
Raybiotech Inc Human Sp1 Activity Assay Kit, 1 Plate Kit
RayBio® Human Sp1 Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical Sp1 binding sequences to semi-quantitatively detect active Sp1 in lysates or nuclear extracts. Dry ice shipment (additional fee).
Eurofins Discoverx PathHunter eXpress CCR5 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0224E2CP0L)
The PathHunter CCR5 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL3/MIP-1α) based activation of CCR5 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCR5 and EA-tagged β-Arrestin-2. Activation of CCR5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human CD80/B7-1 ASY
The R-PLEX Human CD80 (B7-1) Assay Kit from Meso Scale Discovery measures CD80 levels in human serum plasma and cell culture supernatants using electrochemiluminescence (ECL) detection It offers high sensitivity broad dynamic range and precision The kit includes a 96-well plate with capture and detection antibodies buffers blocker solutions calibrator and controls Sensitivity is 0 3 pg/mL with an upper quantification limit of 10 000 pg/mL suitable for biomarker discovery and immunology research
Meso Scale Discovery RPLEX HUM IL28BIFN 3 ASY
The R-PLEX Human IL-28B (IFN-lambda3) Assay is a multiplex immunoassay kit from Meso Scale Diagnostics that enables the quantitative measurement of human interleukin 28B (IL-28B) also known as interferon lambda 3 (IFN-lambda3) This assay utilizes MSD s electrochemiluminescent detection technology and is provided in a 96-well plate format The assay has a detection range of 0 03-1000 pg/mL and requires 50 L of sample per well This product can be used to measure IL-28B levels in serum plasma and cell culture supernatants IL-28B is a cytokine that has antiviral activity and is involved in immune response against viruses